【摘要】 目的:研究TGF-β1对成牙本质细胞系MDPC-23细胞中Sm ads mRNA表达的影响,探讨成牙本质细胞内Sm ads分子基因表达调控机制。方法:培养MDPC-23细胞,细胞分为5组,分别用TGF-β1刺激0、0.5 h、1.5 h、4 h、24 h,提取总RNA。用半定量RT-PCR观察Sm ad 2、Sm ad 3和Sm ad 7 mRNA表达变化。结果:MDPC-23细胞表达Sm ad 2、Sm ad 3和Sm ad 7 mRNA。在TGF-β1作用24 h内,MDPC-23细胞内Sm ad 2 mRNA表达水平无明显变化。在TGF-β1作用4 h后,Sm ad 3 mRNA表达水平下调。Sm ad 7 mRNA水平在TGF-β1作用下1.5 h达到最高峰,以后逐渐下降。结论:在成牙本质细胞内,TGF-β1可能通过不同的方式调控其细胞内信号分子Sm ad 2、Sm ad 3和Sm ad 7的表达,从而使成牙本质细胞对Sm ad介导的TGF-β1信号得到精确调控。更多还原

【Abstract】 AIM:To investigate the effect of TGF-β1 on endogenous Smads in odontoblast cell line MDPC-23 in vitro,and to explore the regulatory mechanism of endogenous Smads expression in odontoblast cells.METHODS:Total RNA was extracted from MDPC-23 cells treated by TGF-β1 for 0,0.5 h,1.5 h,4 h and 24 h.The semi-quantitive RT-PCR was used to investigate the change of Smad 2,Smad 3,and Smad 7 mRNA levels.RESULTS:Smad 2,Smad 3 and Smad 7 mRNA were expressed by MDPC-23 cells.The level of Smad 2 mRNA had no significant difference after treatment by TGF-β1 during 24 h.The level of Smad 3 mRNA was down-regulated after treatment by TGF-β1 for 4 h.The mRNA level of Smad 7 reached a peak after treatment by TGF-β1 for 1.5 h.CONCLUSION:TGF-β1 may regulate the expression of Smad 2,Smad 3 and Smad 7 in a different way,in order to control the intensity and duration of Smad-mediated signaling in odontoblast cells.更多还原