【摘要】 目的探讨检测腹水脱落细胞端粒酶活性对良恶性腹水鉴别诊断的价值。方法收集各种类型腹水脱落细胞,用聚合酶链反应端粒重复扩增(PCR-TRAP)银染法检测端粒酶活性,并与细胞学检查及腹水肿瘤标志物检查结果进行对比分析。结果脱落细胞端粒酶活性检测对恶性腹水诊断的灵敏度为77.33%,显著高于脱落细胞学检查(37.33%)及肿瘤标志物检查(58.67%),特异度和细胞学检查差异无统计学意义。结论腹水中脱落细胞端粒酶活性检测可作为临床良恶性腹水鉴别诊断依据之一。更多还原

【Abstract】 Objective To assess whether telomerase activity in ascitic exfoliative cells can be used as a parameter for the differential diagnosis of benign and malignant ascites. Methods Telomerase activity in the cells from various kinds of ascitic fluid was detected by PCR-TRAP and compared with the cytological diagnosis and tumour marks. Results The result showed that the overall sensitivity of the telomerase assay in detecting malignant ascites was superior to that of cytology examination and total tumour marks assay(77.33% versus 37.33% and 58.67%, respectively), whereas the specificity of the telomerase assay and cytology examination was comparable. Conclusions Telomerase assay in exfoliated cells is useful in differentiating between malignant and benign ascites.更多还原