【摘要】 目的探讨联合p504S、CK34βE12、p63检测在前列腺良、恶性病变鉴别诊断中的应用。方法采用微波S-P免疫组化方法,观察p504S、CK34βE12、p63在前列腺良、恶性病变中的表达情况。结果大多数良性前列腺增生的腺泡和导管周围可见连续的CK34βE12和p63表达,少数呈间断表达,但p504S均皆呈阴性;高级别上皮内瘤CK34βE12、p63呈不连续表达,p504S呈弱阳性或阴性;5例非典型腺瘤性增生,CK34βE12、p63均呈阳性,仅1例p504S局灶弱阳性;40例前列腺癌,CK34βE12、p63均呈阴性,p504S均呈阳性;前列腺癌Gleason分级与p504S免疫表达无关。结论联合检测p504S、CK34βE12、p63可提高前列腺癌诊断的准确率,尤其适合于体积较小的前列腺穿刺标本进行良、恶性鉴别。更多还原

【Abstract】 Objective To evaluate the value of p504S,CK34βE12 and p63 immunostaining for the diagnosis of benign and malignant lesions of the prostate. Methods Expression of p504S、CK34βE12 and p63 in benign and malignant lesions of the prostate was observed by microwave SP immunohistochemical method. Results Continuous positive expression of CK34βE12 and p63 was found in the acini and ducts of most benign prostatic hyperplasia, and few cases showed interrupted expression, but p504S staining presented negative reaction; Interrupted expression of CK34βE12 and p63 was found in high prostatic intraepithelial neoplasia cases, and was weekly positive or negative for p504S; CK34βE12 and p63 staining was positive in five atypical adenomatous hyperplasia cases, one case presented focally positive reaction by p504S; CK34βE12 and p63 staining was negative in forty prostatic carcinoma, but positive by p504S;The gleason histologic grading of prostate carcinoma was negatively related to expression of p504S. Conclusion Combined detection of p504S、CK34βE12 and p63 can be most helpful for diagnosis of prostate carcinoma, especially in needle biopsy specimen.更多还原