【摘要】 利用ITS的通用引物(ITS5-ITS4)对云南的美味牛肝菌(Boletusedulis)子实体的DNA进行PCR扩增,扩增产物回收后直接测序。序列的聚类分析表明,在ITS1-5.8SrDNA-ITS2区域,云南的美味牛肝菌与欧洲的夏牛肝菌(B.aestivalis)和铜色牛肝菌(B.aereus)同源性较高,但在ITS2区域夏牛肝菌和铜色牛肝菌分别有一段美味牛肝菌没有的大小分别为73bp和26bp的特征序列。更多还原

【Abstract】 A pair of general primer (ITS5-ITS4) was used to test in amplification of internal transcribed spacer (ITS) region of chromosomal DNA of fruiting bodies of Boletus edulis in Yunnan.The DNA amplification fragment was purified with DNA Gel Extraction Kit and sequenced by CEQ8000.The result of phylogenetic analyses based on ITS1-5.8S-ITS2 sequences showed that B.edulis in Yunnan had high homology with B.aestivalis and B.aereus in Europe.73?bp and 26?bp DNA characteristic fragments differing from all B.edulis were found in the ITS-region of B.aereus and B.aestivalis respectively.更多还原