【摘要】 目的探讨实验性脊髓损伤中低氧诱导因子1(αHIF-1α)调控的两种血管活性因子诱导型一氧化氮合酶(iNOS)和血管内皮生长因子(VEGF)在损伤脊髓中的表达趋势及其意义。方法将SD大鼠随机分为7组,分别为损伤后12h、1d、2d、3d、1周、2周以及正常对照组,各损伤组以脊髓压迫模型致伤。采用免疫组化方法,观察伤后各损伤脊髓中iNOS和VEGF的表达情况。结果损伤脊髓中iNOS和VEGF的表达都显著增加,它们的表达都在伤后2￣3d达到高峰,以后逐渐下降。结论HIF-1α调控的iNOS和VEGF在损伤脊髓中的表达明显增高,这应是HIF-1α激活下游基因转录的结果,从而使血管扩张,微循环重建,这是损伤脊髓组织对缺血缺氧的一种适应。更多还原

【Abstract】 Objective To investigate the expressional pattern of iNOS and VEGF,which are regulated by HIF-1α,and its significance in spinal cord injury(SCI).Methods Random-bred male Sprague-Dawley rats were prepared for SCI models.After receiving compressive injury at T10,spinal cord was sampled at different courses from 12 hours to 2 weeks after injury and the iNOS and VEGF regulated by HIF-1α were tested with enzyme histochemistry.The data were statistically analyzed.Results In normal spinal cord,the expressions of iNOS and VEGF were found at relatively low level.Since 2 days to 3 days after SCI,the expressions of iNOS and VEGF increased significantly till two weeks(P<0.05).Conclusion These results indicate that the significant increases of expressions of iNOS and VEGF exist in all types of cells in injured spinal cord,and are the result that HIF-1α enhances the transcript of target genes.The dilation of microvessels and revasculization in injured spinal cords should be the most important methods which alleviate hypoxia and ischemic damage in acute spinal injury.更多还原