【摘要】 研究了固相萃取(SPE)富集和预分离,快速分离柱高效液相色谱(HPLC)快速测定枸杞样品中类胡萝卜素的新方法;枸杞样品中的类胡萝卜素用W aters Sep-Pak C18固相萃取小柱预分离,然后以ZORBAX Stab leBound(4.6 mm×50 mm,1.8μm)快速分离柱为固定相,甲醇-四氢呋喃为流动相分离,V(甲醇)∶V(四氢呋喃)=80∶20,用二极管矩阵检测器检测,检测波长为450 nm,枸杞样品中的几种类胡萝卜素在5 m in内可达到基线分离,比常规方法节约时间75%左右。方法标准回收率为95%~103%,RSD为1.9%~2.6%。更多还原

【Abstract】 A rapid column high performance liquid chromatography method for the determination of carotenoids from medlar was studied.Carotenoids were extracted from medlar with tetrahydrofuran（THF） by ultrasonic oscillating,pre-separated by solid phase extraction with Waters Sep-Pak C₁₈cartridge and isolated on a ZORBAX Stable Bound（4.6×50 mm,1.8μm） rapid analysis column with methanol-tetrahydrofuran（80∶ 20,v/v） as mobile phase at a flowrate of 1.5 mL/min,and monitored with photodiode array detector at 450nm.Carotenoids were separated in 5min and 75% of time was saved compared with the traditional method.The recoveries of carotenoids are 95¹03%,and the relative standard deviations are 1.9².6%.更多还原