【摘要】 建立1个同时测定大黄及其制剂中的芦荟大黄素、大黄酸和大黄素含量的反相高效液相色谱法.采用Waters Spherisorb ODS2(5μm,Φ4.6 mm×250 mm)色谱柱,以体积比为85∶15的甲醇-0.1%磷酸溶液为流动相,流速为1.5!mL/min,柱温为40℃,检测波长为430 nm.在上述色谱条件下,芦荟大黄素、大黄酸和大黄素在10 min内得到了有效的分离和检测.方法简便、快速、灵敏、线性范围宽,可用于大黄及其制剂中芦荟大黄素、大黄酸和大黄素的定量测定.更多还原

【Abstract】 Development of a method for the determination of aloe-emodin,rhein and emodin in rheum and rheum-containing preparations by the reversed phase-high performance liquid chromatography(RP-HPLC).The separation and determination was carried out in the Waters Spherisorb ODS2 column(5?μm,4.6?mm i.d.×250?mm)at column temperature of 40?℃ using methanol-0.1% phosphoric acid(85∶15,V/V) as mobile phase with the flow rate of 1.5?mL/min and detective wavelength of 430?nm.Aloe-emodin,rhein and emodin could be separated and determined availably within 10 minutes under the chromatographic conditions selected.The method developed in this paper,which is simple,rapid,sensitive and of wide linear range,can be used for the determination of aloe-emodin,rhein and emodin in rheum and rheum-containing preparations.更多还原