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E物质对红叶石楠“红罗宾”组培苗生根及相关酶活性的影响
Effects of Substance E on Rooting and Activities of Correlative Enzymes of Photinia fraseri "Red Robin" in Vitro
【摘要】 通过对与生根密切相关的酶(POD,IAAO,PPO)活性的测定,发现E物质的添加与否对红叶石楠生根苗中的POD,IAAO,PPO活性存在很大差异。在添加有E物质的培养基上,酶活性均表现为易生根的变化规律;而在未添加E物质的培养基上,酶活性均表现为生根难的变化趋势。从而进一步从生理生化的角度论证了E物质添加与否,直接影响到红叶石楠的生根率、出根时间、整齐度以及出瓶时间。
【Abstract】 In this study,the results showed that,whether E substance was added in the basal medium or not,it had strong influence on rooting of Photinia fraseri in vitro and activities of some enzymes related to rooting(POD,IAAO and PPO);the activities of all tested enzymes of Photinia fraseri cultured in the medium added with substance E took on the change law of rooting easily,while all the tested enzymes’ activities of Photinia fraseri in the medium without substance E displayed the change tendency of being unfavorable to rooting,even to difficult rooting.As a result,from the point of physiological and biochemical study,it was further proved that whether substance E existed in the medium or not had direct influence on the rooting rate,rooting time and uniformity,and transplanting time of plantlets of Photinia fraseri in vitro.
【Key words】 Photinia fraseri "Red Robin"; in vitro; rooting; substance E; enzymes’ activities;
- 【文献出处】 云南农业大学学报 ,Journal of Yunnan Agricultural University , 编辑部邮箱 ,2006年03期
- 【分类号】S687
- 【被引频次】10
- 【下载频次】111