【摘要】 目的:研究神经生长因子(Nerve Growth Fac-tor,NGF)和神经节苷脂GM1(Ganglioside GM1)对2,5-己二酮(2,5-HD)中毒的保护作用。方法:将细胞随机分为对照组和实验组,对照组于正常培养基生长,实验组以2,5-HD、NGF和GM1处理。采用Giemsa染色法在倒置显微镜下观察细胞形态,MTT法检测细胞存活率,流式细胞计数法检测细胞凋亡率。结果:2,5-HD处理后,细胞损伤存活率降低(P<0.01),凋亡率上升(P<0.01);以NGF和GM1预处理的细胞显示出明显的抗损伤性,细胞存活率提高(P<0.05),凋亡率下降(P<0.05),且在联合时较单独应用效果更显著(P<0.01)。结论:2,5-HD抑制PC12细胞存活和增殖,诱导细损伤和凋亡。NGF和GM1对2,5-HD中毒性PC12细胞显示出协同保护作用。更多还原

【Abstract】 AIM: To investigate whether NGF and GM₁ have synergistic protective effect on 2,5-HD intoxication in PC12 cells.METHODS: Cells were divided into control group and treated groups at random.Control group was maintained in normal medium;treated groups were exposed to 2,5-HD with or without NGF,GM₁.The cells’ morphological changes were observed by inverted microscope after being dyed with Giemsa.The cell viability was determined by MTT assay.The apoptotic ratio was determined by flow cytometry（FCM）.RESULTS: PC12 cells which was treated by 2,5-HD injury aggravated,the cell viability decreased（P<（0.01））,and the apoptotic ratio increased（P<（0.01））.PC12 cells pretreated with NGF and GM₁ obviously resisted injury,the cell viability increased（P<（0.05））,apoptotic ratio decreased（P<（0.05））.Combination of NGF and GM₁ afforded additional protection that was greater than that of either agent individually（P<（0.01））.CONCLUSION: 2,5-HD inhibited the survival,induced apoptosis and injury of PC12 cells.NGF and GM₁ showed synergistic protective effect on 2,5-HD intoxication in PC12 cells.更多还原