【摘要】 目的:建立兔血浆溴苯腈检测的气相色谱-质谱分析的方法,研究溴苯腈在兔体内的毒代动力学。方法:雄性日本大耳白7只,予30 mg.kg-1溴苯腈原药灌胃,分别于灌胃前及给药后72 h内多点抽取静脉血。用气相色谱-质谱联用法(GC-MS)测定血浆中溴苯腈浓度。结果用DAS软件进行分析,计算毒代动力学参数。结果:兔30 mg.kg-1溴苯腈ig后t1/2ke为11.327±3.043 h,Tmax为5.571±1.134 h,Cmax为109.943±43.486 mg.L-1,AUC0-tn为2275±959 mg.L.h-1,AUC0-∞为2315±980 mg.L.h-1,MRT0-tn为16.9±2.5 h,MRT0-∞为18.1±2.9 h。结论:溴苯腈经口吸收后的毒代过程符合一级吸收一室开放模型,吸收及消除均较慢。本研究采用的气相色谱-质谱联用法检测血浆溴苯腈浓度准确、快速、简便。更多还原

【Abstract】 AIM: To develop a gas chromatography-mass spectrography method for the determination of Bromoxynil in rabbit plasma,and to study the toxicokinetics of Bromoxynil in rabbits.METHODS: Seven male rabbits were involved in the study.Each subject received a single dose of 30(mg·kg^-1) Bromoxynil tested formulation.Blood samples were conducted consequently within 72 hours.The plasma Bromoxynil concentrations were determined by GC-MS.The plasma Bromoxynil concentration were calculated by ADS and calculated the toxicokinetics parameters.RESULTS: After a single dose of 30(mg·kg^-1) tested formulation,the oxicokinetics parameters of Bromoxynil were as following: t_1/2ke was 11±3 hours,T_max（5.6） ±（1.2） hours,C_max 110±43(mg·L^-1),AUC_0-tn 2275±959(mg·L^-1·h^-1) and AUC_0-∞ 2315±980(mg·L^-1·h^-1),MRT_0-tn（16.9）±（2.5） hours,and MRT_0-∞（18.1）±（2.9） hours.CONCLUSION: After 30(mg·kg^-1) ig administration demonstrates that plasma Bromoxynil concentration against time profile is fitted well to the one-compartment open model with 1st order absorption.Absorption and elimination of Bromoxynil in rabbits were all slow.The method for determination of Bromoxynil concentration in plasma by GC-MS is simple,sensitive,and accurate.更多还原