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金钗石斛茎段诱导原球茎增殖的研究

Studied on Multiplication of Dendrobium nobile protocorm-like-bodies(PLBS)

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【作者】 蒋波陶李华梁泽华何小燕贝学军黄基全

【Author】 JIANG Bo, TAO Li-hua, LIANG Ze-hua, HE Xiao-yan, BEI Xue-jun, HUANG Ji-quan(1.Associate professor ,Department of Chemistry and Biology, Yulin Teachers’ College, Yulin Guangxi 537000) (2.Assistent,dept.of Chemistry and Biology,Yulin Teachers’ College, Yulin Guangxi 537000) (3.Student,dept.of Chemistry and Biology,Yulin Teachers’ College, Yulin Guangxi 537000)

【机构】 玉林师范学院化学与生物系玉林师范学院化学与生物系 副教授广西玉林537000助教广西玉林537000本科生广西玉林537000

【摘要】 以金钗石斛茎段诱导出健壮的原球茎为材料,研究培养基中不同激素组合及不同有机物质对原球茎增殖的影响.结果发现以MS+2mg/L6-BA+0.5mg/LNAA的增殖效果最好,50d后统计,其增殖倍数在6倍以上;在添加不同有机物质的研究中,发现10%椰汁有利于金钗石斛原球茎的增殖,而10%香蕉汁则有利于原球茎的分化.

【Abstract】 How different hormone combination and different organism affaect the multiplication of PLBS which induced by Dendrobium nobile stems is investigated.The best multiplication media is MS+2mg/L6-BA+0.5mg/LNAA and the multiplication factor is 6 times or more after 50 days .Adding different organic matter in a different study,the result shows that 10% coconut milk is conducive to multiplication of PLBS and 10% banana milk is conducive to division of PLBS.

【关键词】 金钗石斛原球茎增殖
【Key words】 Dendrobium nobileprotocorm-like bodymultiplication
【基金】 玉林师范学院科研项目(2006YJYB52)
  • 【文献出处】 玉林师范学院学报 ,Journal of Yulin Teachers College , 编辑部邮箱 ,2006年05期
  • 【分类号】S567.239
  • 【被引频次】5
  • 【下载频次】251
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