【摘要】 目的:探讨细胞外基质(extracellularmatrix,ECM)与激光原位角膜磨削术(laserinsitukeratomileusis,LASIK)术后角膜瓣下上皮细胞植入引起角膜混浊的关系。方法:健康成年大耳白兔随机取1只眼为实验眼,另1只眼为对照眼。做以鼻侧为基底的角膜瓣,机械刮取瓣周围角膜上皮细胞,并植于瓣下,每日裂隙灯观察上皮植入及角膜混浊情况,进行HE染色及免疫组化研究。结果:术后植入上皮处裂隙灯下可见角膜瓣下混浊;HE染色可见层间上皮细胞生长;免疫荧光显示,在正常角膜组织中I、Ⅲ型胶原在基质中排列规则,IV型胶原在基底膜呈强荧光,术后1周起围绕植入上皮周围见Ⅲ、IV型胶原异常强荧光染色。结论:基底膜主要成分IV型胶原在层间的异常沉积是LASIK术后上皮植入角膜混浊的主要原因,Ⅲ型胶原的过量表达也是造成角膜混浊的原因之一。更多还原

【Abstract】 Purpose:To observe the expression of extracellular matrix in cornea and its relationship with corneal opacity after epithelial ingrowth in LASIK. Methods: Corneal flap was made on rabbit eyes, and epithelial cells mechanically scraped from surroundings the flap were implanted underneath. The corneas were harvested 1,3,7 and 30 days following surgery, histological and immunohistochemical examination was performed. Results: On slit-lamp examination, corneal opacity was observed in the area where epithelial cells were implanted. Histological study revealed clusters of epithelial cells at the flap and stroma bed interface. One week and one month post-operation intense immunoreactivity for collagen type IV was detected at the perimeters of the intrastromal epithelial island, but not in the ordinary interface area, strong staining for collage type Ⅲ was also observed around the epithelial islands. Conclusion: The marked deposition of collagen type IV surrounding the ingrowth epithelial cells indicated that it might be the essential component of interface haze in epithelial ingrowth after LASIK.更多还原