【摘要】 目的研究胃癌患者血清肿瘤型M2-丙酮酸激酶(tM2-PK)的含量与临床病理学参数的关系。方法运用ELISA法定量检测tM2-PK在胃癌、胃溃疡、及正常人血清中的含量。结果胃癌患者血清tM2-PK增高,明显大于胃溃疡(P<0·05)与正常人组(P<0·01)。肿瘤≥5cm者明显高于<5cm者(P<0·05);TNMⅢ,Ⅳ期者明显高于Ⅰ,Ⅱ期者(P<0·01);而淋巴结转移情况、肿瘤胃壁浸润深度及组织学分级对tM2-PK水平的影响无明显差异。以大于15U/ml为阳性者,胃癌组阳性率为64%,胃溃疡组假阳性率为20%,正常对照组假阳性率为6%。结论胃癌肿瘤代谢的改变可产生大量tM2-PK。血清肿瘤型M2-PK水平升高可为肿瘤大小和病期提供依据。更多还原

【Abstract】 Objective To determine the serum level of tumor type M2-pyruvate kinase(tM2-PK) in patients with gastric cancer and to elucidate its relationship with clinical characteristics. Methods The serum tM2-PK levels were measured by quantitative sandwich ELISA in 64 gastric cancer patients,15 gastric ulcer patients and 16 healthy individuals. Results The preoperative serum tM2-PK levels in patients with gastric cancer were significantly higher than those in patients with gastric ulcer(P<0.05) and those in healthy individuals(P<0.01). The bigger tumors (≥5cm) had significant higher tM2-PK than the smaller ones(<5cm)(P<0.05). The levels in the patients of stage Ⅲand stageⅣ were significantly higher than those of stage Ⅰand stageⅡ(P<0.01). No significant difference could be found in different tumor invasive depth,lymph node metastasis and histological types.Taking 15U/ml as the positive result,the positive rate was 64% in gastric cancer patients,and false positive rates were 20% and 6% in gastric ulcer patients and in healthy individuals,respectively.Conclusion With the changing of tumor metabolism,gastric cancer can produce a lot of tM2-PK,which can be measured in serum. The serum levels of tM2-PK may reflect the size and stage of the tumor.更多还原