【摘要】 目的观察FALL-39和头孢他定(CFZ)治疗脓毒血症的疗效。方法从pGEX-1λT-FALL-39转染的E.coliJ M109中提纯FALL-39,检测FALL-39对绿脓杆菌(PAG)的杀菌活性。采用更生霉素(AMD)增强BALB/c小鼠对PAG感染的敏感性,建立脓毒血症模型,观察FALL-39和CFZ治疗脓毒血症的疗效。结果FALL-39分子量约5kDa,纯化量约600~650μg,对PAG的最低抑菌浓度(MIC)、最低有效浓度(MEC)、最低杀菌浓度(MBC)分别为25、100、50μg/ml。盐水组、脓毒血症组、CFZ治疗组、FALL-39治疗组和CFZ+FALL-39治疗组小鼠死亡率分别为0%、75%、62·5%、37·5%和12·5%。结论FALL-39治疗脓毒血症比CFZ更为有效,两者联用效果则更佳。更多还原

【Abstract】 Objective To investigate the effects of FALL-39 peptide and cefatazigine on treating sepsis.Methods Purified FALL-39 was extracted from Escherichia coli JM109 transfected by the pGEX-1λT-FALL-39 recombinant vector.The antibacterial activity of FALL-39 was verfied by measuring the MIC,MEC,MBC for P.aeruginosa.The infectional sensitivity of BALB/c mice for P.aeruginosa was induced by employing actinomycin D and the actinomycin-sensitized BALB/C murine sepsis model was constructed.The treatment effects of FALL-39 peptide and cefatazigine on BALB/C murine sepsis model were investigated.Results The molecular magnitude of FALL-39 was identified approximate 5kDa.About 600～650μg of highly purified FALL-39 was obtained.The antibacterial activity of FALL-39 was determined,and its minimal effective concentration(MEC),minimal inhibitory concentration(MIC),and minimal bactericidal concentration(MBC) were 25,100,and 50μg/ml, respectively. Death rates of saline,sepsis,CFZ,FALL-39 recombinant peptide and CFZ+FALL-39 subgroups were 0%,75%,62.5%,37.5%,and 12.5%,respectively.Conclusion FALL-39 recombinant peptide is more effective than ceftazigine in treating sepsis and the efficacy of combining ceftazigine with FALL-39 recombinant peptide in treating sepsis is more than FALL-39 or ceftazigine alone.更多还原