【摘要】 目的探讨N-乙酰半胱氨酸(NAC)对重症急性胰腺炎(SAP)的治疗作用及作用机制。方法54只SD大鼠随机分为假手术(SO)组、SAP组及NAC处理组。采用胰胆管逆行注射5%牛磺胆酸钠诱导大鼠SAP模型,NAC处理组于模型制作前30 min腹腔内注射NAC(200 mg/kg)进行预处理。分别观察各组大鼠血浆淀粉酶、TNF-α、IL-6、丙二醛(MDA)、胰腺及肺组织病理以及组织髓过氧化物酶(MPO)的变化。结果SAP组血浆淀粉酶、TNF-α、IL-6、MDA、胰腺及肺组织MPO均较SO组明显升高(P<0.05),NAC处理组各检测指标均较SAP组显著降低(P<0.05),胰腺及肺组织病理损伤明显减轻。结论NAC可通过清除氧自由基、减少TNF-α和IL-6的产生等机制对SAP具有重要的治疗和保护作用。更多还原

【Abstract】 Objective To investigate the therapeutic effect and mechanism of N-acetylcysteine(NAC) on severe acute pancreatitis(SAP).Methods Fifty-four SD rats were randomized into sham operation(SO) group,SAP group and NAC-pretreated group.The model of SAP was(induced) by retrograde injection of 5% sodium taurocholate into the bili-pancreatic duct.NAC-pretreated group was given 200mg/kg NAC intraperitoneally 30min before inducing SAP.The levels of(amylase,)TNF-α,IL-6,malondialdehyde(MDA) in plasm,and myeloperoxidase(MPO) in the pancreas and lung were measured.The histopathological changes of pancreatic and pulmonary tissues were evaluated.(Results)The levels of amylase,TNF-α,IL-6,MDA in plasm,and the MPO in the pancreas and lung were significantly increased in SAP group compared with those in SO group(P<0.05).(Compared) with SAP group,the indexes were significantly decreased in NAC-pretreated group(P<0.05),the severity of pancreas and lung injury also significantly decreased in NAC-pretreated group.(Conclusion)NAC may have a therapeutic effect on SAP by cleaning oxygen free radicals and decreasing the production of TNF-α and IL-6.更多还原