【摘要】 目的分析大肠癌患者含表皮生长因子和卵泡抑素结构域的跨膜蛋白(TPEF)基因甲基化状态,寻找新的早期诊断大肠癌分子标志物。方法常规方法提取大肠癌标本DNA,采用甲基化特异性PCR技术分析TPEF基因甲基化状态。结果32例大肠癌手术切除标本,24例检测到异常TPEF基因甲基化(75%),而10例正常组织标本无一例检测到异常甲基化。结论大肠癌患者TPEF基因甲基化为一频繁发生事件,可望成为大肠癌早期诊断的一个标志物。更多还原

【Abstract】 Objective The methylation status of transmembrane protein endothalial factor(TPEF) gene was analyzed for finding a new molecular diagnostic marker of colorectal tumor.(Methods)Genomic DNA was isolated from tumor samples using a standard procedure,and TPEF methylation status was analyzed with methylation-specific PCR.Results Abnormal TPEF methylation was detected in 24(75%)from 32 samples(obtained) from patients underwent operation,but no one in 10 normal samples.Conclusion Abnormal methylation of TPEF gene is a frequent event and may become a promising diagnostic marker for early detecting colorectal carcinoma.更多还原