【摘要】 目的检测肿瘤坏死因子相关凋亡诱导配体(TRAIL)及其受体在原发性肝癌(PHC)及癌旁组织中的表达。方法收集PHC及癌旁组织各30例,采用半定量RT-PCR方法,对TRAIL及其受体的表达作半定量检测。结果(1)30例癌组织及癌旁组织中均有TRAIL及其死亡受体4(DR4)、死亡受体5(DR5)、“诱骗”受体1,2(DcR1、DcR2)的表达。(2)TRAIL、DcR1及DcR2在癌组织中的表达量较之癌旁组织明显降低(P<0.01),而DR4、DR5的表达量则高于癌旁组织;(3)PHC组织中4种受体之间,DR4及DR5的表达量明显高于DcR1及DcR2,且PHC组织中4种受体间的表达量存在相关性。结论PHC及其癌旁组织中均存在TRAIL及其受体的表达,且有表达量的差异。更多还原

【Abstract】 Objective To investigate the expression of tumor necrosis factor-related apoptosis-inducing ligand(TRAIL) and its receptors in patients with primary hepatic carcinoma(PHC).(Methods)TRAIL and its receptors were assayed by semiquantitive RT-PCR in 30 PHC specimens,and 30 specimens of adjacent non-malignant liver tissues.Results TRAIL and its receptors were(detectable) in all of the tumors and adjacent non-malignant tissues.Expressions of TRAIL,DcR1,DcR2 were lower,but those of death receptor 4(DR4) and DR5 were higher in hepatoma tissues than those in the adjacent non-malignant tissues.Expressions of DR4 and DR5 were higher than those of DcR1 and DcR2 in hepatoma tissues,which were not significantly different in the adjacent non-malignant tissues.There was a correlation among the expression TRAIL receptors.Conclusion TRAIL and its receptors areexpressed in both hepatoma tissues and adjacent non-malignant tissues with different(expression) levels.更多还原