【摘要】 目的从肺腺癌A549细胞对拉伸应力响应时释放出的细胞形变调整行为,解读肿瘤细胞骨架异常与其生物学行为的相关性。方法使用单向等轴应变加载装置对培养的肺腺癌A549细胞施以周期性基底拉伸加载,通过计算机图像处理并与对照组进行比较。结果(1)A549细胞的形态在加载中有明显的变化,铺展面积明显增大,细胞外形变得非常不规则,即,细胞不规则度增加;(2)与对照组比较,加载组细胞细胞核的铺展面积增大,拉伸引起细胞核形态的变化比较小,加载组与对照组细胞核的形态差别不大;(3)周期性拉伸引起了细胞取向的调整,细胞调整到与主应变近似垂直的方向,许多细胞拉长首尾连成一线,线的取向趋于与主应变方向垂直。结论A549细胞的细胞骨架各组份间对应力的响应协同反应下降;肺腺癌A549细胞对应力方向的呼应尚属正常。更多还原

【Abstract】 Objectives To unscramble the relation between biological action and cytoskeleton’s abnormity in tumor fromthe phenomenon that when A549 cell respons to strain morphological changes are induced. Methods A549 cell waschosen and an unidirectional equiaxial strain loading device in vitro was set up to apply cyclic stretch to the substrate ofthe cells, with the computer image processing and mathematical statistics. Results The results of the experiment wereanalyzed and compared with the contrast. It’s demonstrated that: 1. During the stretch course, the cells have presentedmorphologic changes clearly. The ratio of the long axis and short axis had increased obviously going with the cells spreadarea increased; 2. Nucleus spread area increased, the modality alteration which was induced by substrate stretch wasnot patency; 3. Cyclic stretch made orientation of the A549 cells change to the vertical direction of the stretch. Cells werestretched longer and linked head to tail in the form of a line. Conclusion: 1.The respondence of cytoskeleton componentsof A549 (mainly include cytoskeleton of membrane, cytoplasm and nucleus) to stretch are separate and unsynchronized;2. The respondence of A549 to the stretch is normal.更多还原