【摘要】 目的探讨重组日本血吸虫信号蛋白14-3-3(rSj14-3-3)间接ELISA用于血吸虫病免疫诊断的价值。方法表达并利用纯化的rSj14-3-3建立间接ELISA法,比较其与可溶性虫卵抗原(SEA)ELISA和间接血凝试验(IHA)检测急慢性日本血吸虫病患者的阳性率及其它寄生虫患者和正常人血清的交叉反应。结果rSj14-3-3-ELISA、SEA-ELISA和IHA3种方法的敏感性分别为90·8%、94·2%和90·0%,特异性分别为87·0%,84·8%和84·8%,差异无显著性(P>0·05)。结论rSj14-3-3抗原间接ELISA法具有可用于日本血吸虫病免疫诊断的价值。更多还原

【Abstract】 Objective To evaluate the recombinant signaling protein 14-3-3 of schistosoma japonicum (rSj14-3-3) on the immunodiagnosis of schistosomiasis by indirect ELISA. Methods The rSj14-3-3 expression in E. coli was induced and purified with affinity chromatography. The coated antigen concentration and sera dilution were optimized by checkerboard experiment. sera of patients with acute and chronic schistosomiasis and other sera were detected with rSj14-3-3 and soluble eggs antigen of schistosoma japonicum (SEA) by indirect ELISA and IHA,respectively. Results The positive rates of indirect ELISA in 64 cases of acute and 56 of chronic schistosomiasis were 92.2% and 89.2% using rSj14-3-3, whereas 95.3% and 92.8% with SEA, and 96.8 % and 82.1 % in IHA. Cross-reaction was noted in 9.4% of normal sera tested by rSj14-3-3 antigen and SEA, compared with 15.6% of false positive in IHA. There were no statistically significant difference of the results among rSj14-3-3, SEA-ELISA and IHA (P>0.05). Conclusion The indirect ELISA with rSj14-3-3 antigen shows higher sensitivity and specificity in SEA for diagnosis of schistosomiasis.更多还原