【摘要】 目的培养经骨保护素OPG修饰的大鼠骨髓基质细胞系并检测其表达能力,鉴定表达产物,为基因工程技术治疗牙周病提供物质基础。方法利用已构建的pSecTag-OPG真核分泌表达穿梭载体,用脂质体法将目的基因转染至大鼠骨髓基质细胞,应用免疫组织化学和Westernblot方法证实转染的细胞表达骨保护素。结果经免疫组织化学和Westernblot方法检测,在转染OPG的大鼠骨髓基质细胞中有OPG表达。结论本实验成功培养出分泌表达OPG的大鼠骨髓基质细胞系。更多还原

【Abstract】 Objective To study the ability of expression and secretion of eukaryotic expression plasmid of human osteoprotegenrin by detecting its expression in rat MSC cell lines so as to inform gene engineering therapy of periodontitis. Methods The constructed recombinant plasmid was transformed into rat MSC cell line and OPG expressed in rat MSC cell line was determined using immunohistochemistry and western blot. Results Recombinant plasmid pSecTag-opg was transformed into rat MSC cell line,and OPG-expression cell line of rat MSC was selected and confirmed by immunohistochemistry and western blot.Conclusion OPG high-expression rat MSC cell line is determined.更多还原