【摘要】 目的对2000-2004年广州市登革热(DEN)病原体分离鉴定及流行特点分析,为该病的诊断、预防和治疗提供依据。方法采用间接酶联免疫(ELISA)法、免疫斑点法、免疫层析法、对疑似患者的血清特异性抗体IgM、IgG进行检测。用C6/36细胞对早期病例血清进行病毒分离,以间接免疫荧光(McAb-IFA)、RT-PCR方法鉴定。RT-PCR法检测成蚊、蚊幼。结果病毒分离株经用单克隆抗体间接免疫荧光(McAb-IFA)、RT-PCR检测鉴定为Ⅰ级登革病毒。检出幼蚊有登革病毒Ⅰ型。结论2001-2004年广州市的登革热流行是由Ⅰ型登革病毒感染所致,2002年出现暴发与流行并延伸到2003年。更多还原

【Abstract】 Objective To isolate and identify the pathogen of dengue fever in Guangzhou city and analyze its serology and pathogeny c haracteristics from 2000 to 2004, and so as to provide scientific bases for diagnosis, prevention and treatment of dengue fever. Methods Antibodies IgM, IgG in serum samples from patients with suspected dengue fever were detected by ELISA、ELISPOT and Immunochromatography. Serum samples from patients with early stage dengue fever were used to isolate virus with C6/36 cell line and the positive serums were identified by McAb-IFA and RT-PCR. Mosquitoes were detected dengue virus. Results The virus isolated from the patients’ serums and mosquitoes were confirmed as dengue virus type 1 by McAb-IFA and RT-PCR. Conclusions The pathogen of dengue fever occurred in Guangzhou from 2000 to 2004 was the dengue virus type 1. Dengue fever broke out and spread in 2002 and extended into 2003.更多还原