【摘要】 设计一对PCR引物,寡聚核苷酸序列的上游引物为5-′gCg,AAT,TCA,TCC,CAC,gTg,CCT,gC-3,′下游引物为5-′gAg,AAT,TCC,Tgg,ggA,ggg,ACC,TT-3′。经68℃退火及30轮循环的PCR程序后,分别克隆了来自我国甘肃、青海和云南地区牦牛β乳球蛋白基因的5′调控区1 447 bp的DNA片段。将该片段从琼脂糖凝胶中回收,克隆在pMD18-T Vector后进行序列测定。序列分析结果表明,甘肃牦牛与青海牦牛该序列的核苷酸同源性为98.13%,与云南牦牛该序列的核苷酸同源性为97.65%,青海牦牛与云南牦牛该基因片段的核苷酸序列同源性为99.45%。更多还原

【Abstract】 In this study a pair of primers for PCR were designed,the oligonucleotides were as follows: the upstream primer,5′gCg,AAT,TCA,TCC,CAC,gTg,CCT,gC-3′,downstream primer,5′-gAg,AAT,TCC,Tgg,ggA,ggg,ACC,TT-3′.After 30 cycles of PCR procedures with annealing temperature at 68 ℃,a fragment of the 5′ regulatory region of β-lactoglobulin gene(1 447 bp) were amplified from Gansu,Qinghai and Yunnan yaks respectively and collected from agarose gel.Then the fragments were cloned into pMD18-T vector and sequenced.The sequencing results show that the homology in the 5′regulatory region of β-lactoglobulin gene between Gansu and Qinghai yaks is 98.13%,that between Gansu and Yunnan yaks 97.65%,and that between Qinghai and Yunnan yaks as high as 99.45%.更多还原