【摘要】 应用扩增片段长度多态性(AFLP)技术,从64个引物对中筛选出9个,分别对37份鸭茅种质进行扩增,共得到400条清晰条带。其中多态性带336条,平均多态位点水平为84.0%,表现出较高的多态性。利用NTSYS-pc软件计算种质间的遗传距离,变化范围为0.0692~0.4214。用UPGMA方法进行聚类分析,在遗传相似系数为0.81水平上37份鸭茅种质可分为8个类群。用EIGEN方法进行主坐标分析,建立种质间相互关系的二维图。各种质在二维图中的分布与UPGMA聚类基本吻合。从分子水平分析,鸭茅遗传变异与染色体倍性和地理分布密切相关。更多还原

【Abstract】 Thirty-seven accessions of wild Dactylis glomerata L.were used in assessment of genetic diversity by means of amplified fragment length polymorphism(AFLP).Nine primer pairs were selected from 64 preparations and then used to analyze the plant materials.A total of 400 DNA fragments were amplified,among which 336(84.0%) were polymorphic.Genetic distance,from 0.0692~0.4214 among accessions,was calculated by the NTSYS-pc software.A dendrogram was constructed using UPGMA based on genetic distance matrix.Eight clusters were defined at the genetic similarity of 0.81.A two-dimensional PCO plot representing relationships among accessions was also produced by EIGEN,demonstrating the same distribution pattern as from the cluster analysis.Based on molecular analysis,the genetic variation of D.glomerata germplasms was closely associated with ploidy levels and geographical distributions.更多还原