【摘要】 根据MTNR1A基因在GenBank中的已知DNA序列设计了2对引物,采用PCR-SSCP技术在一个大白猪和长白猪群体中进行单核苷酸多态性(single nucleotide polymorphism,SNP)检测,发现了一个SNP位点,并对其不同基因型个体PCR回收产物进行测序。测序结果发现该SNP是由于在+159碱基处(GenBank中序列)发生了G→A的同义突变而引起的。并对该SNP与产仔数进行了关联分析,结果表明该SNP对产仔数的影响不显著。更多还原

【Abstract】 Two pairs of primers were designed based on the known sequence in GenBank for amplification of MTNR1A gene in a Large White and a Landrace herd.Using PCRSSCP(single strand conformation polymorphism),we found a single nucleotide polymorphism(SNP) within the product amplified from the first pair of primers.PCR products from randomly selected different genotypes were sequenced after were recovered and purified.Results revealed a synonymous single base mutation(G→A) at +159bp(sequence numbering based on Genbank accession number U73326) for the BB genotype.When analyzed for association with litter size traits,this MTNR1A SNP was found to have no significant effect on litter size traits.更多还原