【摘要】 筛选汉族群体中具有多态性的Y染色体双等位基因标记并获取其群体遗传学数据。采用片段长度差异等位基因特异性PCR和PAGE技术对武汉地区160名男性汉族无关个体的23个Y染色体双等位基因标记(M7,M9,M50,M88,M89,M95,M111,M117,M119,M121,M122,M134,M159,M164,M175,M214,LINE1,MSY2,RPS4Y711,SRY+465,IMS-JST164520,IMS-JST021354和IMS-JST003305)进行分型。除M50、M159和M164外,其余20个标记在武汉汉族群体中均具有遗传多态性,其基因多样性(GD)范围为0.0126~0.4855,共检出35种不同单体群组合(Hg1~35),单体群多样性(HD)为0.9471。表明20个Y染色体双等位基因标记组成的单体群具有较高的遗传多样性,在法医学应用和群体进化研究中具有较高的实用价值。更多还原

【Abstract】 To search polymorphic Y chromosome biallelic markers in Chinese Han population,and obtain their population genetic data.Genotyping of 23 biallelic markers on human Y chromosome(M7,M9,M50,M88,M89,M95,M111,M117,M119,M121,M122,M134,M159,M164,M175,M214,LINE1,MSY2,RPS4Y₇₁₁,SRY₊₄₆₅,IMSJST164520,IMS-JST021354 and IMS-JST003305) were carried out in a sample of 160 unrelated Chinese male individuals living in Wuhan using fragment length discrepant allele specific PCR（FLDAS-PCR） and PAGE technique.In all 23 biallelic markers,genetic polymorphism were identified for 20 loci in Wuhan Han population except for M50,M159 and M164,and the ranges of gene diversity（GD） were 0.0126～0.4855.A total of 35 different haplogroups（Hg1～35） were observed and the haplogroup diversity（HD） was 0.9471.The haplogroups formed by 20 biallelic markers are highly polymorphic,and can be used in forensic science and population evolution studies.更多还原