【摘要】 次黄嘌呤鸟嘌呤磷酸核糖转移酶(Hprt)参与嘌呤核苷酸的补救合成。采用RACE技术克隆了黄鳝的次黄嘌呤鸟嘌呤磷酸核糖转移酶基因,它的全长cDNA为1 452 bp,预测编码218个氨基酸,与人类、小鼠、鸡和斑马鱼等脊椎动物Hprt氨基酸序列之间的同源性超过76.7%。基于该基因氨基酸序列构建了进化树,显示与斑马鱼Hprt基因更同源。RT-PCR表明黄鳝Hprt基因在多种组织中广谱表达,表明黄鳝该基因在功能和进化上的保守性。更多还原

【Abstract】 The enzyme hypoxanthine-guanine phosphoribosyltransferase(HPRT) plays an important role in the purine salvage pathways.Full-length cDNA of swamp eel(Monopterus albus,the rice field eel) Hprt gene was obtained by the RACE method.The cDNA sequence was 1 452 bp in length,which encoded a protein of 218 amino acids.Amino acid identities of Hprt between swamp eel and other vertebrates including human,mouse,chicken and zebrafish were more than 76.7%.Phylogenetic tree analysis based on the amino acid sequences showed that Hprt of swamp eel and zebrafish was in one clade.RT-PCR analysis showed ubiquitous expression pattern of swamp eel Hprt in adult tissues,suggesting that it has a constitutive function and is conserved during evolution.更多还原