【摘要】 ZNF268是一个典型的KRAB类锌指基因,含有24个C2H2型锌指基序和一个KRAB功能域,它在人胚胎发育早期表达,并与胚胎肝脏的发育相关。通过免疫组化的方法,发现ZNF268在3~5周龄人胚的造血干细胞中有表达;进一步通过RT-PCR的方法检测发现ZNF268在健康成人全血细胞中也有表达,并且表达有4个差异剪接本,从中克隆得到两个新的ZNF268差异剪接本ZNF268c和ZNF268d。推测ZNF268和血细胞生长发育相关。更多还原

【Abstract】 ZNF268 gene encodes a typical KRAB-containing zinc finger protein,which has an amino-terminal KRAB domain and 24 carboxyl-terminal C₂H₂ zinc finger motifs.There is evidence that ZNF268 is expressed in human early embryos,and that it played a role in the development of human fetal liver.In this study,we used immunohistochemistry to show that ZNF268 was also expressed in hematopoietic stem cells in 3～5 week-old human embryos.ZNF268 expression was also detected in the blood cells from 7 healthy adult donors by RT-PCR.Furthermore,4 alternative transcripts were detected in the 7 samples.By cloning and sequencing,two novel transcripts,ZNF268c and ZNF268d were identified.We predict that there is association between ZNF268 and the development of hematopoietic cells.更多还原