【摘要】 采用PCR方法从小麦(Triticum aestivumL.)新品种“川麦42”中克隆得到一个低分子量谷蛋白亚基(LMW-GS)新基因,暂命名为LMWCM42-1。该基因编码区全长846 bp,编码281个氨基酸,具有LMW-GS基因的典型结构特征。推导氨基酸序列比较显示,尽管LMWCM42-1与已知LMW-GS高度相似,但在N-末端重复区部分重复单元以及C-末端区中仍存在明显差异。聚类分析表明,LMWCM42-1可能是由Glu-D3位点编码的。更多还原

【Abstract】 Using PCR-amplification method,the coding sequences of a novel low-molecular-weight glutenin(LMW-GS) gene,LMWCM42-1,was isolated from the genomic DNA of wheat(Triticum aestivum L.) variety ’Chuanmai 42’.The full coding region of this gene consisted of 846 nucleotides,and encoded a protein with 281 amino acids.LMWCM42-1 is a typical low-molecular-weight glutenin subunit(LMW-GS) gene.In spite of high similarity with the known LMW-GSs,LMWCM42-1 was quite different from the known LMW-GS in the N-terminal,central repetitive domain and C-terminal domain.It was found that LMWCM42-1 was located within the Glu-D3 locus by phylogenetic analysis.更多还原