【摘要】 以油樟带芽茎段为材料,在初代培养中进行了不同表面消毒方式、不同年龄、不同季节的外植体污染率试验。结果表明,Ca(ClO)2和HgCl2的配合使用有利于污染率的降低;当年生枝条的污染率低于二年生枝条。3月采集的材料污染率低于10月。这为增加油樟组培中无菌苗的得率提供了指导,也对其它木本植物组培的研究有一定的意义。更多还原

【Abstract】 The different season and age stem sections with buds as explants are tested in contamination rates,and the tests of different sterilizhing modes to different explants are also done.The results show that H_gCl₂ supplemented with C_a（ClO）₂ is to step down the contamination rate; the contamination rate from current growth shoot is lower than that from the second year growth shoot; the contamination rate of explants collected in March is lower than that in October. It provides a guidance for increasing germ free shoot yield in tissue culture of cinnamomun longepaniculatun（Gamble）N.Chao. It is also some significance in tissue culture of other woddy plants.更多还原