【摘要】 文中利用MEMS工艺在硅基底上制备铂微电极芯片,并采用双层电化学结构进行抗体的固定,用于人免疫球蛋白IgG的安培酶免疫检测。第一层为聚吡咯过渡层,第二层为抗体与聚邻苯二胺的共聚层。该传感器工作电压-0.3V,检测下限5 ng/mL,线性范围5~355 ng/mL,响应时间3~5 min,具有制备简便、响应快、下限低、试剂用量少、微型化、便于集成等优点。更多还原

【Abstract】 A micro Pt amperometric immunosensor on silicon wafer based on MEMS was fabricated to detect human IgG.Antibodywas immobilized usingtwo-layer polymers.Firstly polypyrrole was electropolymerized onthe electrode asthetransitionlayer.Then anti-body and poly o-phenylenediaminewas electropolymerized.Withthe working potential of-0.3 V,the immunosensor displayed a goodlinear dose response behaviorfor humanIgGconcentrations between5 and 355 ng/mL.Alowdetectionlimit of 5ng/mLandresponsetimeof 3 ~5 minute were achieved.This micro amperometric immunosensor has many advantages suchas easyfabrication,fast responsetime,lowdetectionlimit,little reagent,micro bulk and easyintegration withIC.更多还原