【摘要】 目的建立五更清肠溶片中补骨脂素的含量测定方法。方法HypersilODS C18柱(250 mm×4.6 mm,5μm),流动相为甲醇-水(45∶55),检测波长为246 nm。结果补骨脂素在12.5~125μg.m l-1范围内,浓度与峰面积值呈良好线性关系,r=0.9996,平均加样回收率为98.12%,RSD=1.8%。结论本方法简便,重复性好,可用于五更清肠溶片的质量控制。更多还原

【Abstract】 Objective To establish a method for determinating the content of Psoralen in Wugengqing Enteric-coated Tablets.Method HPLC method was used,the separation was performed on Hypersil ODS C₁₈（250 mm×4.6 mm,5 μm）,methyl-water（45∶55） as mobile phase and quantified by ultraviolet detection with wavelength at 246 nm.Result The range of calibration curve was 12.5～125 μg·ml^-1,r=0.9996,average recovery rate was 98.12%,and RSD was 1.8%.Conclusion This method is simple,accurate and reproducible for the quality control of Wugengqing enteric-coated tablet.更多还原