【摘要】 目的探讨FeC l3诱导的大鼠动脉血栓形成模型血浆TXA2、PG I2含量及抗凝、纤溶活性的变化。方法以FeC l3外敷诱导大鼠左侧颈总动脉血栓形成,测定血浆TXA2和PG I2稳定代谢产物TXB2、6-keto-PGF1α含量及t-PA、PAI-1、PLG、AT-Ⅲ和PC活性。结果浓度为2.16 mol.L-1的FeC l3可诱导大鼠颈总动脉闭塞性血栓形成,且该模型血浆TXB2含量升高(P<0.05),6-keto-PGF1α含量降低(P<0.05);血浆t-PA和PLG活性降低(P<0.05),PAI-1活性无变化(P>0.05),t-PA/PAI-1比值降低(P<0.05);血浆AT-Ⅲ活性降低(P<0.01),PC活性轻微降低(0.10>P>0.05)。噻氯匹定可抑制FeC l3诱导的血栓形成,但对TXB2和6-keto-PGF1α无影响,表明其抗栓作用不是通过抑制血小板TXA2生成而实现的;噻氯匹定还可升高血浆t-PA活性,可能系该药通过抑制血栓形成,减少了血浆t-PA消耗所致。而该药对AT-Ⅲ和PC活性无影响。抗凝血药低分子肝素(LMWH)也可抑制血栓形成,该药对TXB2和6-keto-PGF1α无影响。LMWH可升高血浆t-PA活性和t-PA/PAI-1比值,可能与其促进血管内皮细胞释放t-PA有关。LMWH还可使该模型血浆AT-Ⅲ活性降低,这是由于LMWH与AT-Ⅲ结合而发挥其抗凝作用,从而使血浆中AT-Ⅲ消耗所致。结论FeC l3可诱导大鼠闭塞性动脉血栓形成,该模型与血小板活化和凝血系统激活有关,同时该模型存在抗凝蛋白活性降低和纤溶活性降低的病理改变。更多还原

【Abstract】 Aim To investigate changes of TXA₂ and PGI₂ content and anticoagulative and fibrinolytic activity in the plasma in a rat model of topical FeCl₃-induced carotid artery thrombosis.Methods Thrombi were induced by topical ferric chloride to left carotid artery in rats.The content of TXB₂ and 6-keto-PGF_1α(stable metabolite of TXA₂ and PGI₂, activity of t-PA,PAI-1,PLG,AT-Ⅲ and PC in the plasma in the model of thrombosis were determined.Results Occlusive thrombi were induced by applying FeCl₃(2.16 mol·L^-1) to left carotid artery.The content of TXB₂ was increased markedly（P<0.05）,and the content of 6-keto-PGF₁α was lower remarkably in the rat plasma（P<0.05）.Activities of t-PA,PLG and AT-Ⅲ were lower in the plasma（P<0.05,P<0.01）,activity of PAI-1 had no change（P>0.05）,but t-PA/PAI-1 ratio reduced（P<0.05）.Activity of PC dropped slightly（0.10>P>0.05）.Ticlopidine could inhibit thrombosis induced by ferric chloride,but it did not influence TXB₂ and 6-keto-PGF₁α content and activities of AT-Ⅲ and PC in the plasma.Ticlopidine could enhance activity of t-PA in the plasma.It may be related to the decrease of t-PA consumption by inhibiting thrombosis.Antithrombosis action of ticlopidine may not be concerned to inhibiting production of TXB₂ of platelet.LMWH could inhibit thrombosis also,but it did not influence TXB₂ and 6-ketoPGF_1α content in the plasma.LMWH could enhance activities of t-PA and t-PA/PAI-1 ratio,which may be related to the promoting release of t-PA in vascular endothelial cells.LMWH could reduce activity of AT-Ⅲ,which may be concerned with combination of LMWH and AT-Ⅲ result in AT-Ⅲ consumption.Conclusion Ferric chloride may induce occlusive thrombosis in rats.Thrombosis may be associated with activation of platelet and blood coagulation system,lower of anticoagulative protein and fibrinolytic activity.更多还原