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阿伦磷酸钠对小鼠骨髓基质细胞成骨和成脂分化的影响
Effects of Alendronate sodium on osteogenic and adipogenic differentiation of bone marrow stromal cells
【摘要】 目的在离体条件下研究阿伦磷酸钠对小鼠骨髓基质细胞(BMSCs)成骨和成脂分化的影响。方法采用四甲基偶氮唑盐(MTT)法检测阿伦磷酸钠对骨髓基质细胞增殖的影响,碱性磷酸酶(ALP)活性测定法检测阿伦磷酸钠对骨髓基质细胞(MSCs)成骨分化的影响,油红O染色形态学及定量测定法检测阿伦磷酸钠对骨髓基质细胞成脂分化的影响。结果1×10-10,1×10-9,1×10-8,1×10-7,1×10-6和1×10-5mol.L-1的阿伦磷酸钠可以促进小鼠原代骨髓基质细胞增殖,抑制骨髓基质细胞的成骨分化及成脂分化。结论阿伦磷酸钠对骨质疏松症的预防和治疗作用可能通过抑制骨髓基质细胞的成脂分化,进而减少脂肪细胞细胞因子的分泌而抑制破骨细胞的形成、激活以及骨吸收功能。
【Abstract】 Aim To study the effects of alendronate sodium on osteogenic and adipogenic differentiation of primary mouse bone marrow stromal cells(MSCs).Methods The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide(MTT),alkaline phosphatase(ALP) activity assay and Oil Red assay were used to investigate the effects of alendronate sodium on the growth,ostogenesis and adipogenesis of MSCs,respectively.Results Alendronate sodium promoted the proliferation of primary mouse bone marrow stromal cells,inhibited osteogenic differentiation and adipogenic differentiation of MSCs at concentrations of 1×10-10,1×10-9,1×10-8,1×10-7,1×10-6 and 1×10-5 mol.L-1,respectively(P<0.05).Conclusion Alendronate sodium may have preventive and therapectic effects on osteoporosis by inhibiting adipogenesis of MSCs,thorough reducing cytokines secretion by adipocytes to inhibit formation,activation and bone resorption of osteoclasts.
【Key words】 alendronate sodium; bone marrow stromal cells; osteogenic differentiation; adipogenic differentiation;
- 【文献出处】 中国药理学通报 ,Chinese Pharmacological Bulletin , 编辑部邮箱 ,2006年06期
- 【分类号】R965
- 【被引频次】23
- 【下载频次】452