【摘要】 目的研究人尿萃取物CDA-Ⅱ(cell d ifferentiation a-gent-Ⅱ,又名尿多酸肽)对人胶质瘤细胞SWO-38抑制增殖及诱导分化作用。方法应用MTT法、集落形成试验检测CDA-Ⅱ对SWO-38细胞增殖的影响;通过光镜观察和免疫组化鉴定细胞分化;利用裸鼠抑瘤实验观察CDA-Ⅱ抗胶质瘤生长的作用。结果一定浓度的CDA-Ⅱ可使SWO-38细胞增殖与集落形成均明显受抑,并呈剂量依赖性。经1 g.L-1CDA-Ⅱ处理后,光镜观察发现SWO-38细胞胞体变大,核浆比减小,突起增多,表现出向成熟的星形细胞分化现象。免疫组化显示胶质纤维酸性蛋白(glial fibrillary ac id ic prote in,GFAP)表达增强。体内抑瘤试验见高、低剂量CDA-Ⅱ均可抑制裸鼠移植瘤生长,抑瘤率为79.94%、42.77%(P<0.05,n=10)。结论CDA-Ⅱ不仅能够抑制人胶质瘤SWO-38细胞的生长,而且能诱导SWO-38细胞分化,是一种很有应用前景的诱导分化剂。更多还原

【Abstract】 Aim To investigate the effect of CDA-Ⅱ（Uroacitide） which is a healthy human urine extraction on the proliferation inhibition and cellular differentiation of human glioma cells SWO-38.Methods Inhibition of SWO-38 cell proliferation was determined using MTT assay and colony formation assay.Cellular differentiation was determined with light microscopy observation and immunohistochemistry method.Nude mice were injected sc with cells SWO38,and after tumor growing daily ip with CDA-Ⅱ for 28 days,observe the inhibitory effect.Results After SWO-38 cells treated with various concentration of CDA-Ⅱ,cell proliferation and colony formation were significantly inhibited in a dose-dependent manner.Light microscopy revealed that CDA-Ⅱ could induce SWO-38 cells differentiate toward astrocyte with increased cytoplasm and cytoplasmic processes and decreased nucleus/cytoplasm rate.Furthermore,SWO-38 cells expressed more GFAP protein.CDA-Ⅱ at dosage of 0.5 g·kg^-1·d^-1 and 2 g·kg^-1·d^-1 with ip administration for 4 weeks significantly suppressed the growth of human glioma cells SWO-38 in nude mice,the inhibition rates were 42.77% and 79.94%,respectively（P<0.05,n=10）. Conclusion CDA-Ⅱ not only inhibited growth of human glioma cells SWO-38,but also induced differentiation.It was a potential induce-differentiation reagent.更多还原