【摘要】 目的探讨多重荧光原位杂交(M-FISH)技术检测慢性淋巴细胞白血病(CLL)复杂核型异常(CCA)的价值。方法应用M-FISH技术对6例常规细胞遗传学(CC)显示CCA的CLL进行研究。结果M-FISH检测仅有1例检出了CCA克隆;1例检出非复杂核型的异常克隆及1个CCA细胞;1例检出3个不同的染色体变异细胞。其余3例M-FISH未检出异常核型分裂象。6例CLL的核型分析中共发现平衡易位10种;非平衡易位9种,其中der(14)t(16;14;9)是既往文献未报道的易位。结论对伴有CCA的CLL以M-FISH技术可以发现和纠正CC漏检及误检的染色体异常。单个细胞核型异常在CLL中多见。更多还原

【Abstract】 Objective To investigate the value of multiplex fluorescence in situ hybridization(M-FISH) in the detection of complex chromosomal abnormalities(CCAs) of chronic lymphocytic leukemia(CLL).Methods M-FISH was used to study 6 cases of CLL with CCAs identified by conventional cytogenetics(CC).Results M-FISH found only 1 case with CCA clone,but didn′t find abnormal clones in other 3 cases.10 balanced and 9 unbalanced translocations were detected in CLL,of which der(14)t(16;14;9) had never been reported.Conclusion For CLL patients,M-FISH can find or correct the missing or misidentified chromosomal abnormalities CC failed to discover.Single-cell abnormalities were frequent in CLL.更多还原