【摘要】 目的探讨钙黏素23(CDH23)基因多态性与噪声性听力损失易感性关系。方法采用病例对照研究方法对254名接触噪声工人进行研究,聚合酶链反应-限制性片断长度多态性方法检测CDH23基因单核苷酸多态性位点rs1227049和rs1227051的多态性。结果rs1227049位点在听损组CC、CG和GG基因型频率分别为39.4%、15.0%和45.6%,等位基因C和G的频率分别为46.9%和53.1%;对照组3种基因型的频率分别为51.2%、19.7%和29.1%,等位基因C和G的频率分别为60.9%和39.1%。rs1227051位点在听损组TT、CT和CC基因型频率分别为82.7%、17.3%和0,等位基因T和C的频率分别为91.3%和8.7%。对照组3种基因型的频率分别为89.0%、11.0%和0,等位基因T和C的频率分别为87.0%和13.0%。两位点的基因型分布及其等位基因频率在听损组和对照组之间差异均无统计学意义(P>0.05)。采用多因素Logistic回归分析对个体间年龄、工龄和累积噪声暴露量等因素进行校正后,也未发现两个单核苷酸位点任一基因型的改变会引起噪声性听力损失的危险度有显著性提高(P>0.05)。结论本研究还不能认为CDH23基因rs1227049和rs1227051两个单核苷酸多态性位点是噪声性听力损失易感性的危险因素。更多还原

【Abstract】 Objective To explore the relationship between CDH23 genetic polymorphism and susceptibility to noise-induced hearing loss.Methods A case-control study was conducted among 254 workers exposed to occupational noise.The genetic polymorphism of two single nucleotide loci rs1227049 and rs1227051 in CDH23 were detected with the method of polymerase chain reaction-restriction fragment length polymorphism.Results Frequencies of genotypes CC、CG and GG in rs1227049 locus were 39.4%、15.0% and 45.6% respectively in 127 cases of the noise-induced hearing loss(NIHL)group and 51.2%、19.7% and 29.1% respectively in 127 cases of the control group,and frequencies of alleles C and G in the same locus were 46.9% and 53.1% respectively in the NIHL group and 60.9% and 39.1% respectively in control group.Frequency of genotypes TT、CT and CC in the rs1227051 locus were 82.7%、17.3% and 0,respectively in the NIHL group and 89.0%、11.0% and 0,respectively in the control group,and frequencies of alleles T and C in the same locus were 91.3% and 8.7% respectively in the NIHL group and 87.0% and 13.0% respectively in control group.No significant higher risk for hearing loss was found in those with any of the genotypes of the two loci(P>0.05),adjusted by age,year of noise exposure and cumulative noise exposure with multivariate logistic regression analysis.Conclusion It doesn’t show that genetic polymorphism of the rs1227049 and rs1227051 loci in gene CDH23 might be a susceptible factor for noise-induced hearing loss.更多还原