【摘要】 目的探讨GSTM1和GSTT1基因多态性与噪声性听力损失易感性的关系。方法采用病例对照研究方法和多重聚合酶链反应(PCR)技术检测听损组123(118)例和对照组123(114)例的GSTM1和GSTT1基因缺失型频率,以2检验检测听损组和对照组基因型频率的差异。结果GSTM1基因在听损组和正常组的缺失率分别为69.1%和56.1%,差异具有统计学意义(P<0.05)。GSTM1(-)基因型携带者发生噪声性听力损失的危险性是携带GSTM1(+)基因型者的1.75倍。GSTT1基因在听损组和正常组的缺失率为50.8%和57.9%,差异没有统计学意义(P>0.05)。联合分析表明,携带GSTM1(-)和GSTT1(-)基因型者发生噪声性听力损失的危险性虽稍高于携带GSTM1(+)和GSTT1(+)基因型者(OR=1.11,2=0.16,P>0.05),但差异无统计学意义,由此认为GSTM1和GSTT1基因之间可能不存在联合作用。结论GSTM1基因缺失可能是发生噪声性听力损失的易感因素之一。更多还原

【Abstract】 Objective To explore the relationship between GSTM1 and GSTT1 genetic polymorphisms and susceptibility to noise-induced hearing loss.Methods A case-control study was conducted among 123(118)cases and 123(114)controls.The genetic polymorphisms of GSTM1 and GSTT1 were detected with the method of multiplex polymerase chain reaction.2 test was used to detect the frequency of genotype of GSTM1 and GSTT1 in the cases and the controls.Results The frequency of the GSTM1 deletion was 69.1% and 56.1%,respectively in the cases and the controls,and their difference was statistically significant(P<0.05).Persons with GSTM1(-)had higher risk of noise-induced hearing loss than those with GSTM1(+).The frequency of the GSTT1 deletion was 50.8% and 57.9%,respectively in the cases and the controls,and their difference was not statistically significant(P>0.05).The results showed that the genes of GSTM1 and GSTT1 had no combined action,those with GSTM1(-)and GSTT1(-)genotype had high risk of developing noise-induced hearing loss than those with GSTM1(+)and GSTT1(+)(OR=1.11,2=0.16,P>0.05).Conclusion GSTM1 gene deletion may be one of risk factors contributing to development of noise-induced hearing loss.更多还原