【摘要】 目的:研究雷公藤多苷抗炎作用的安全范围及抗炎机制。方法:用二甲苯致小鼠耳肿胀模型和小鼠雷公藤多苷灌胃给药3 d的毒性实验,用Bliss法分别计算ED50,ED95,ED99和LD50,LD5,LD1,计算安全范围指标治疗指数(TI),安全系数(SF)和可靠安全系数(CSF);用放免法测大鼠血清中前列腺素E2(PGE2)的量, 用放免法测环氧化酶-2(COX-2)活性。结果:雷公藤多苷对小鼠有明显的抗炎作用,且呈良好的量效关系; 抗炎作用的TI,SF和CSF分别为6．1,0．9和0．4;雷公藤多苷对大鼠关节肿胀和血清PGE2有抑制作用,对 COX-2无明显的影响。结论:雷公藤多苷抗炎作用安全范围窄,小剂量多次给药相对安全。雷公藤多苷抗炎机制可能不是通过影响COX-2活性来发挥作用的。更多还原

【Abstract】 AIM: To research the margin of safety and mechanism of Tripterygium wilfordii polyglycosidum (TwP) in resisting inflammation. METHODS: Creation of mouse model swelling ear by dimethylbenzene and calculation of TwP toxic experiment by 3 d of mouse feeding were carried out to obtain median effective dose (ED50) ,ED95,ED99 and median lethal dose (LD50) ,LD5 and LD1 through Bliss method;with simultaneously gaining of TI,SF and CSF indexes. Serum PGE2 consentration and radioactivity intensity (DPM value)of rats were sampled with through radioimmunoassay (RIA) method. RESULTS: The anti-inflammation effect of TwP on mice was obviously significant with good quantity-efficiency relationship. A anti-inflammaory efficiency of TI, SF and CSF for TwP were 6.1,0.9 and 0.4 respectively.TwP lessened rats’ adjuvant arthritc in flammation and reduced PGE2 in serum,but revealed no conspicuous change on COX-2. CONCLUSION: TwP can significantly resist inflammation, but with narrow safety margin;small spliting doses would be more preferable.The anti-inflammaory mechanism of TwP may not be brought into play via influencing COX-2.更多还原