【摘要】 目的:研究群体感应系统对铜绿假单胞菌生物膜形成的影响。方法:采用生理盐水-吸痰管系统进行生物膜的培养,3天后用扫描电镜观察PAO1、PAO1lasIrhlI、PAO1lasRrhlR形成生物膜的情况。结果:PAO1野型可形成较厚、有孔状通道的生物膜,而PAO1lasIrhlI基因缺陷型和PAO1lasRrhlR基因缺陷型形成的生物膜明显稀薄,未能形成成熟的生物膜结构。结论:表明PAO1野型与lasIrhlI和lasRrhlR基因缺陷型铜绿假单胞菌体外形成生物膜的能力差异有显著性。更多还原

【Abstract】 Objective:To understand the effects of quorum sensing system on P. aeruginosa biofilm formation. Methods:Examination of the biofilm developments of PAO1,lasIrhlI and lasRrhlR mutants in vitro by means of scanning electronic microscopy (SEM) after three days’ incubation.Results:After three days’ incubation,well developed biofilm was observed in PAO1 group,whereas poor biofilm formation was seen in lasIrhlI and lasRrhlR mutant groups.Conclusion:Quorum sensing system is important in the formation of P.aeruginosa biofilm.更多还原