【摘要】 目的:探讨HCV-RNA定量检测和HCV基因分型的临床意义。方法:用荧光定量PCR法检测212例血清HCV-RNA或抗-HCV阳性标本,采用第三代ELISA夹心法检测抗-HCV,全自动生化分析仪测定ALT,应用RT-PCR法对78例标本进行基因分型。结果:在212例标本中HCV-RNA和抗-HCV双阳性的155例,两者阳性符合率73.1%(155/212),并且HCV-RNA载量和抗-HCV(+)例数呈正相关(r=0.92,P<0.05);HCV-RNA载量与ALT异常例数(r=0.57,P<0.05)呈正相关;78例标本丙肝基因分型HCVⅡ/1b型占92.3%。结论:HCV-RNA定量或抗-HCV检测均有一定的局限性,同时应用HCV-RNA定量或抗-HCV检测使诊断更为准确;我国人群丙肝感染以Ⅱ/1b型为主,基因分型诊断为选择药物治疗提供科学依据。更多还原

【Abstract】 Objective:To study the clinical significance of the quantitative detection and genotype of HCV-RNA. Methods:Fluorescent quantitative PCR methods were used to detect 212 cases of anti-HCV or HCV-RNA positive samples,the concentrations of anti-HCV and ALT were detected by the third generation ELISA and automatic biochemistry analyzer. The HCV-RNA genotypes of 78 cases were detected by RT-PCR. Results:Among 212 samples,HCV-RNA(+)/anti-HCV(+) both positive results were 155 cases. Positive coincident rate was 73.1%(155/212).It was positive correlation between quantity of HCV-RNA and the number of anti-HCV positive samples(r=0.92,P<0.05). It was positive correlation between the quantity of HCV-RNA and the number of samples with abnormal ALT(r=0.57,P<0.05).78 samples were genotyped:92.3% were HCVⅡ/1b. Conclusion:There was limitation in both HCV-RNA and anti-HCV methods. It was more accurately to using two methods to diagnosing HCV. People in our country were mainly infected by HCV Ⅱ/1b.Diagnosis of HCV genotype could provide scientific basis for drug selection.更多还原