【摘要】 目的:优选一种适用献血员梅毒检测的试验方法。方法:采用梅毒特异性抗体的双抗原夹心法(ELISA)对献血员进行抗体检测,并与RPR法和TRUST法检测结果进行比较,3种方法检测结果有反应性的标本,再用TPHA法进行确证。结果:ELISA法有反应性率1.69%(184/10884),RPR法有反应性率1.23%(134/10884),TRUST法有反应性率1.30%(141/10884);ELISA法与TPHA法符合率97.8%(180/184),RPR法与TPHA法符合率71.2%(131/184),TRUST法与TPHA符合率75.5%(139/184)。进行统计学处理,ELISA法与RPR法、TRUST法具有非常显著性差异。ELISA法与TPHA法无显著性差异。结论:ELISA法优于TRUST法和RPR法具有较高的灵敏度和特异性,适合献血员的梅毒项目的优选试验方法,有利于控制梅毒的输血传播途径。更多还原

【Abstract】 Objective:To select a suitable method for the detection of anti-TP antibody in blood donors. Methods:Anti-TP antibody was detected by ELISA of double sandwich,and compare with the results of RPR and TRUST. Then TPHA method was employed to confirm the reactive samples. Results:The reactive rate of ELISA was 1.69%(184/10 884),and RPR was 1.23%(134/10 884),while TRUST was 1.30%(141/10 884);The coincidental rate between ELISA and TPHA was 97.8%(180/184),between RPR and TPHA was 71.2%(131/184),while that between TRUST and TPHA was 75.5%(139/184). After statistical analysis,the difference between ELISA and RPR was significant,and so was that between ELISA and TRUST,but there was no obvious difference between ELISA and TPHA. Conclusion:ELISA is better than TRUST and RPR,and suitable for the detection of anti-TP antibody in blood donors. Because of its high sensibility and specificity,it is helpful to control the route of syphilitic transmission in blood transfusion.更多还原