【摘要】 为了探讨提高机采血小板血浆中的含氧量对血小板功能的影响,将机采血小板样品分为实验和对照2组。实验组在无菌条件下提高机采血小板血浆中含氧量(溶解氧)后,两组同时放置(22±2)℃水平振荡的血小板振荡仪器中保存。分别在血小板保存0、1、2、3、4、5天检测血小板量数量、血小板聚集反应功能、血小板液乳酸含量和血小板CD62p表达量。结果显示2组的血小板数量、血小板聚集反应功能均随保存时间延长而下降;2组间比较,血小板数量无显著性差异(P>0.05),血小板聚集反应功能实验组在保存2-3天明显好于对照组(P<0·05)。2组的血小板乳酸含量、血小板CD62p表达量均随保存时间延长而升高;2组间比较,实验组的血小板乳酸含量和血小板CD62p表达量在保存1-3天时低于对照组(P<0.05);机采血小板的含氧量在0天时实验组显著高于对照组(P<0.01)。结论提高血小板血浆中的含氧量(溶解氧)可弥补保存袋中血小板代谢的供氧不足,提高血小板的有氧代谢和血小板的保存质量。更多还原

【Abstract】 To explore the influence of raising oxygen (dissoved oxygen) content on function of platelet concentrate, the platelet concentrate was prepared by a CS-3000 plus blood cell separator. Experiments were divided into 2 groups: test group and control group. After raising oxygen content in platelet plasma under sterile operation, the platelet samples of two groups were preserved in oscillator with horizondal oscillation at 22±2℃. The platelet count, platelet aggregation rate, lactic acid content and CD62p expression level of platelet were detected on 0,1,2,3,4,5 days of platelet preservation. The results showed that the platelet count and platelet aggregation rate decreased with prolongation of preserved time, while the lactic acid content and CD62p expression level of platelet increased gradually. Compared with control group, there were significant diffrerences in aggregation rate of platelet preserved for 2-3 days, and in CD62p expression level of platelet preserved for 1-3 days, while significant diffrerence was found in lactic acid content of platelet preserved for 1-3 days. It is concluded that raising content of oxygen in platelet plasma can provide more oxygen to compensate oxygen supply deficiency for platelet metabolism and improve the efficiency of platelet oxygenic metabolism and the quality of platelet during preservation.更多还原