【摘要】 本研究探讨在As2O3作用下人淋巴瘤Raji细胞的增殖及VEGFmRNA表达的变化。采用改良MTT方法观察As2O3对Raji细胞的增殖抑制效应;采用半定量RT-PCR方法检测As2O3对Raji细胞VEGF121和VEGF165mRNA表达的影响。结果表明As2O3对Raji细胞的作用表现时间、剂量依赖性的增殖抑制效应,建立回归方程为IR=0.531dose+0.481time(P<0.05)。半定量RT-PCR方法检测显示,Raji细胞同时表达VEGF121和VEGF165两种剪切变异体,二者的表达水平相当(VEGF1211.17±0.14;VEGF1651.31±0.19,P>0.05)。VEGF121和VEGF165mRNA表达量与As2O3作用时间呈负相关关系(rsv121=-0.547,P<0.05;rsv165=-0.632,P<0.05),且VEGF的各异构体对药物的反应具有差异性,VEGF165mRNA表达下调早且有持续性。但在所选浓度组中未发现类似的相关关系(P>0.05)。结论As2O3既可抑制Raji细胞生长增殖,亦可通过下调VEGFmRNA表达而产生抗血管新生效应,且小剂量、长时程给药时结果亦然。更多还原

【Abstract】 This study was aimed to investigate the effects of As₂O₃ on proliferation of B lymphoma Raji cell and to study the expression changes of VEGF mRNA. The modified MTT was adopted to evaluate the effect of As₂O₃ on proliferation of Raji cells. Semi-quantitative RT-PCR was used to detect the expression of VEGF 121 and VEGF 165 mRNA in Raji cells exposed to As₂O₃. The results showed that the As₂O₃ significantly inhibited the proliferation of Raji cells, the relationship between the inhibition rate and the concentrations of As₂O₃ was dose-and time-dependent. The VEGF 121 and VEGF 165 mRNA expressions were found in Raji cells, and both were well-matched in expression levels. After treatment of As₂O₃ at 1 μmol/L for 48 hours, the expression levels of VEGF 121 and VEGF 165 mRNA were significantly down-regulated and demonstrated negative corelation with the time of exposure to As₂O₃. Otherwise some difference were observed in effects of As₂O₃ on VEGF 121 and VEGF 165 , the expression of VEGF 165 mRNA was down-regulated earlyer and longer than that of VEGF 121 , while no similar correlation was found in selected concentration groups. It is concluded that As₂O₃ can significantly inhibit the growth of Raji cells and may exerted its anti-angiogenesis effects by down-regulating the VEGF mRNA expression even in a low concentration for a long term.更多还原