【摘要】 本研究观察右旋柠烯(d-limonene,D-L)对HL-60和K562白血病细胞增殖凋亡的影响,并进一步探讨其作用机制。首先采用碘化丙锭染色法观察右旋柠烯对HL-60、K562细胞增殖的影响,再通过细胞形态学观察、流式细胞术分析、免疫细胞化学半定量测定突变型p53、bcl-2、bax基因的表达水平,系统观察D-L对HL-60、K562细胞体外诱导凋亡的情况。结果表明D-L抑制HL-60和K562细胞的增殖,IC50均为0.75mmol/L,呈剂量时间依赖关系;D-L诱导HL-60、K562细胞凋亡;D-L在诱导HL-60细胞凋亡过程中随作用浓度增加,bcl-2的表达下降;D-L在诱导K562细胞凋亡过程中随作用浓度增加,突变型p53及bcl-2的表达下调,而bax的表达上调。结论D-L抑制HL-60和K562细胞增值并诱导其凋亡;突变型p53,bcl-2,bax参与了D-L诱导凋亡的基因调控。更多还原

【Abstract】 To investigate the effects of D-limonene(D-L) on the cell growth and apoptosis in HL-60, K562 cells and to elucidate its mechanism, the influence of D-L on proliferation of HL-60 and K562 cells was determined by propidium iodide assay, the expression levels of mutant p53, bcl-2, bax gene were detected by cell morphological analysis, flow cytometry and immunohistochemistry staining, the D-L-inducing HL-60 and K562 cell apoptosis in vitro was observed systematically. The results showed that D-L inhibited HL-60 and K562 cell growth in a dose-and time-dependent manner with the IC_ 50 of 0.75 mmol/L similarly, D-L induced apoptosis of HL-60 and K562 cells, and expression of bcl-2 gene was down regulated by D-L in a concentration-dependent manner in HL-60 cells.The bcl-2, mutant type of p53 genes were down regulated while bax gene was up regulated by D-L in a concentration-dependent manner in K562 cells. It is concluded that D-L can inhibit proliferation and induce apoptosis of HL-60 and K562 cells. The bcl-2, mutant type of p53 and bax may be involved in the gene regulation of D-L-induced apoptosis.更多还原