【摘要】 同源盒基因家族成员HOXB4反映原始造血干/祖细胞(PHSC/PHPC)的自我更新和增殖能力。本研究采用实时定量RT-PCR的方法在mRNA水平上检测HOXB4基因的表达,以观察体外扩增脐血CD34+细胞自我更新的水平。结果显示:随着体外培养时间的延长,虽然CD34+细胞数增加,但HOXB4表达下降;周后,HOXB4几乎检测不到,与成熟外周血淋巴细胞表达HOXB4的水平相同;CD34+细胞与骨髓间充质干细胞(BM-MSC)共培养可以减缓CD34+细胞HOXB4表达的下降。结论:脐血CD34+细胞体外扩增过程中自我更新能力逐渐下降。CD34+与BM-MSC共培养有助于减缓体外扩增的CD34+细胞自我更新能力的丧失。更多还原

【Abstract】 HOXB4, a member of homeobox gene family, is closely related to the self-renewing and proliferative ability of primitive hematopoietic stem/progenitor cells(PHSC/PHPC). This study was aimed to investigate the self-renewing level of cord blood progenitor cells (CBPC) expanded in vitro. The HOXB4 expression at mRNA level was assayed by using real time RT-PCR. The results indicated that as culture prolonged, the total cells, CD34~+ cells greatly increased, however the HOXB4 expression gradually declined, even down to undetectable level similar to that of mature lymphocytes. Meanwhile, it was shown that CD34~+ cells co-cultured with bone marrow mesenchymal stem cells (BM-MSC) could abate the decline of HOXB4 expression. It is concluded that the self-renewing potential of CD34~+ cells gradually decreased during expansion in vitro, co-culture with BM-MSC was helpful to CD34~+ cell expansion and slowed the loss trend of its self-renewal.更多还原