【摘要】 目的观察血府逐瘀汤对结扎冠脉所致缺血大鼠心肌细胞凋亡及ICAM-1(intercellularadhesionmolecule-1)表达的影响。方法采用结扎冠状动脉左前降支的方法复制急性心肌缺血模型,运用DNA末端标记法(TUNEL)和免疫组化技术分别观察心肌细胞凋亡及ICAM-1蛋白表达的变化。结果假手术组仅见极少量凋亡阳性细胞;心肌缺血组有明显心肌细胞凋亡增多,与假手术组比较差异显著(P<0.01);血府逐瘀汤组、地奥心血康组心肌细胞凋亡均显著减少,与心肌缺血组比较差异显著(P<0.01,P<0.05)。同时ICAM-1蛋白的阳性表达支持心肌细胞凋亡的情况。结论血府逐瘀汤与地奥心血康可有效抑制心肌细胞坏死凋亡,下调ICAM-1表达,减轻心肌细胞损伤,对缺血心肌有保护作用。更多还原

【Abstract】 Objective To explore the effects of Xiefuzhuyu decoction on myocardial cellar apoptosis and expression of ICAM-1 gene protein in rats of experimental acute ischemic myocardium. Methods The left anterior descending coronary artery was ligated to make a model of acute ischemic myocardium. The changes in myocardial cellar apoptosis and the ICAM-1 gene expression were detected. Results The number of myocardium cellar apoptosis in Xiefuzhuyu group and Diaoxinxiekang group were significantly less than those of ischemic model group respectively. The number of myocyte necrotic cell increased in ischemic group. At the same time, the expression of ICAM-1 supported this condition. Conclusion Xiefuzhuyu decoction and Diaoxinxiekang can protect ischemic myocardium.更多还原