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胶体染料试纸条试剂盒检测曼氏血吸虫病的初步研究

Preliminary study on detection of schistosomiasis mansoni with dipstick dye immunoassay kit

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【作者】 朱荫昌Sohair Hassen何伟曹国群

【Author】 Zhu Yinchang~1, Sohair Hassen~2, He Wei~1, Cao Guoqun~1 (1 Jiangsu Institute of Parasitic Diseases, Jiangsu Provincial Key Lab on Molecular Biology of Parasites, Jiangsu Provincial Key Subject on Parasitic Diseases, Wuxi 214064, China; 2 National Research Center, Cairo, Egypt)

【机构】 江苏省血吸虫病防治研究所、江苏省寄生虫分子生物学重点实验室、江苏省寄生虫病学重点学科埃及国家研究中心江苏省血吸虫病防治研究所、江苏省寄生虫分子生物学重点实验室、江苏省寄生虫病学重点学科 无锡214064无锡214064

【摘要】 目的探索日本血吸虫胶体染料试纸条试剂盒(DDIA)用于检测曼氏血吸虫病的可行性。方法采用日本血吸虫DDIA检测从埃及尼罗河地区曼氏血吸虫病流行区采集的曼氏血吸虫病人血清及当地健康人血清。结果34份曼氏血吸虫病人血清DDIA均呈阳性,14份当地健康者血清均为阴性。与用曼氏血吸虫IHA和ELISA法检测结果相同。结论日本血吸虫DDIA也可用于检测曼氏血吸虫病。

【Abstract】 Objective To evaluate the possibility for detection of schistosomiasis mansoni with dipstick dye immunoassay (DDIA) kit. Methods DDIA kit prepared with Schistosoma japonicum egg antigen was used to detect the sera of schistosomiasis mansoni patients from the endemic areas of Egypt, as well as the sera of healthy people from Egypt as a control group. Results Thirty four sera of schistosomiasis mansoni were positive, but the 14 healthy people sera were negative. The results were the same as with IHA and ELISA of Schistosoma mansoni. Conclusion The dipstick dye immunoassay kit could be applied for detection of schistosomiasis mansoni.

【基金】 江苏省卫生厅重大科研项目(H2006)
  • 【文献出处】 中国血吸虫病防治杂志 ,Chinese Journal of Schistosomiasis Control , 编辑部邮箱 ,2006年06期
  • 【分类号】R532.21
  • 【被引频次】3
  • 【下载频次】66
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