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里氏木霉LW1产纤维素酶的酶学性质

Enzymatic Characteristics of Cellulase Produced by Trichoderma Reesei LW1

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【作者】 王菁莎王颉刘景彬

【Author】 WANG Jing-sha1,WANG Jie1,LIU Jing-bin2(1.Food Science College,Hebei Agriculture University,Baoding 071001,China;2.School of Food Science and Biotechnology,Tianjin University of Science and Technology,Tianjin 300222,China)

【机构】 河北农业大学食品科技学院天津科技大学食品科学与生物技术学院 河北保定071001河北保定071001天津300222

【摘要】 研究了里氏木霉LW1所产纤维素酶的主要酶学性质。结果表明,该纤维素酶的最适温度为50℃,最适pH值为4.8;在30~50℃,pH 4.0~6.0范围内有较高的稳定性;90℃处理15 min,酶粉的CMC酶活和FPA酶活保存率分别为38.66%和52.68%;Ca2+、K+、Na+、Mg2+离子对酶活有激活作用,Mn2+、Zn2+、Cu2+离子有抑制作用。

【Abstract】 Enzymatic characteristics of the crude enzyme from Trichoderma Reesei LW1 were studied.The optimal conditions for enzymatic reaction were 50℃,and pH value were 4.8;showed a high stability at temperature 30~50℃,pH 4.0~6.0;stayed 15 min at 90℃,CMCase could be held 38.66%,and FPase can be held 52.68%;Ca2+、K+、Na+、Mg2+ were the activators and Mn2+、 Zn2+、Cu2+ were the inhibitors of the enzyme.

  • 【文献出处】 纤维素科学与技术 ,Journal of Cellulose Science and Technology , 编辑部邮箱 ,2006年02期
  • 【分类号】O629.8
  • 【被引频次】6
  • 【下载频次】214
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