【摘要】 探讨在不同剂量农用混合稀土“常乐”的作用下,大鼠肝脏的形态、功能改变及其作用机理。以W istar大鼠为对象,用生理盐水和2.0、5.0、20.0m g.kg-1的常乐连续灌胃3个月后,采用生化方法检测了血清中天冬氨酸氨基转移酶(A ST)、丙氨酸氨基转移酶(ALT)、碱性磷酸酶(ALP)、γ-谷氨酰胺转移酶(GGT);应用常规组织学及透射电镜技术观察了大鼠肝脏光镜及电镜形态结构的变化;采用酶组织化学技术检测了肝细胞中琥珀酸脱氢酶(SDH ase)、葡萄糖-6-磷酸酶(G-6-Pase)及三磷酸腺苷酶(C a2+-ATPase)的变化。与对照组比较2.0、5.0m g.kg-1组大鼠肝脏结构、血清酶及SDH ase、G-6-Pase、C a2+-ATPase的活性均无明显改变;20.0m g.k-g 1组肝细胞索排列紊乱,汇管区有炎细胞浸润,糖原减少,线粒体嵴轻度肿胀,胆小管附近有较多电子密度高的致密体及溶酶体,SDH ase、G-6-Pase和C a2+-ATPase活性均明显降低。给予不同剂量的混合稀土,大鼠肝脏出现不同程度的改变,以20.0m g.kg-1组显著。更多还原

【Abstract】 To probe the effects of different dose Changle on morphological change in the liver and their mechanism in rats.120 young Wistar rats were divided into four groups,which were given Changle at 2.0mg·kg^-1,5.0mg·kg^-1,20.0 mg·kg^-1 and the control group with physiological saline respectively for threemonths.Pathological changes of liver were observed by light microscope and transmission electron microscope;The changes of SDHase,G-6-Pase and Ca²⁺-ATPase in rat liver were oberserved byenzyme histochemistry technique;AST（aspartate aminotransferase）,ALT（alanine aminotransferase）,GGT（gamma glutamyltransferase）,ALP（alkline phosphatase） contentsin the serum were measured.Comparing with the control group,in the 2.0 mg·kg^-1,5.0 mg·kg¹group of Changle,there are no obvious changes in hepatocytes,blood serum enzyme,SDHase、G-6-Pase and Ca²⁺-ATPase.In the20.0 mg·kg^-1groupshows injurious effects on the rat hepatocytes,there are decrease of glycogen in the rat hepatocytes,denser matrix of the mitochondria,deformation of the nuclei of some hepatocytes with different degrees and infiltration of inflammatory cells at portal area.The levels of SDHase、G-6-Pase and Ca²⁺-ATPase in rat hepatocytes in the group of 20.0 mg·kg^-1 groupwere reduced obviously.The more the rats are given doses,the more the number of bodies contain high electronic dense gravel-like granules and the secondary lysosomes with dense bodiesaround the bile canaliculus.There are increased changes of GGT in blood serum of 20.0 mg·kg^-1group.Changle can affect structureand function of ratliver.The effects on rat liver are correlativewith different doses of Changle,but with high dose of Changle(20.0 mg·kg^-1).更多还原