【摘要】 研究了不同稀土浓度的镧、钕柠檬酸配合物溶液对纤维素酶水解不同纤维素底物的活性的影响规律,并对其作用机制进行了分析。用沉淀法分别合成了镧、钕柠檬酸配合物;用EDTA络合滴定法分析了镧、钕在配合物中的含量;用红外光谱法对镧、钕柠檬酸配合物进行了表征;用DNS试剂法测定了不同稀土浓度的镧、钕柠檬酸配合物溶液对纤维素酶水解不同纤维素底物的酶活性。结果表明:柠檬酸中羧基脱去质子后与镧、钕离子配位,在合适浓度时,两个配合物对纤维素酶水解滤纸、羧甲基纤维素钠、纤维二糖的活性均有最大的激活作用,钕配合物的激活效果好于镧配合物。更多还原

【Abstract】 The influence of La and Nd complexes with citric acid on the cellulase activity for the different cellulose was studied.The La and Nd complexes with citric acid were synthesized by the deposition method.The content of the La and Nd in the complexes was analyzed by the method of EDTA titration.The complexes coordinates with the carboxyl of citric acid.The activity of the cellulase,which is the quantity（μg） of gained dextrose from the enough cellulose per mg cellulase per minute,was determined with the DNS method.The results reveal that the La and Nd complexes with citric acid respectively could stimulate the cellulase activity and maximize the activity at 1.0×10^-8 g·L^-1 or 1.0×10^-9 g·L^-1,which is the concentration of La³⁺ or Nd³⁺,for the different cellulose,such as filter paper,CM-Cellulose Na and Cellobiose.The influence of Nd complex on the cellulase activity is stronger than that of La complex.更多还原